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Copyright© 2009
the Jensen Lab

Publications

 

Here we have linked the pdf-files of the original articles where we are allowed to do so.

 

 

However, in most cases this is not permitted. Therefore we are providing the link to the article on the journals' websites.

 
|2011|  |2010| |2009| |2008| |2007|  |2006|  |2005|
 

2011

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Pilhofer, M., Ladinsky, M., McDowall, A. W., Petroni, G. and, Jensen, G. J. (2011) Microtubules in Bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton. PLoS Biol (9:12): e1001213. doi:10.1371/journal.pbio.1001213

 

Electron Tomography of Cells

Gan, L., and Jensen, G. J. (2011). Electron Tomography of Cells. Quarterly Reviews of Biophysics, FirstView Article: 1-30. doi:10.1017/S003358351100012

Briegel, A., Beeby, M., Thanbichler, M., and Jensen, G. J.. (2011) Activated chemoreceptor arrays remain intact and hexagonally packed. Mol. Micro. 82(3): 748-757

Draper, O., Byrne, M., Li, Z., Keyhani, S., Barrozo, J., Jensen, G. J., and Komeili, A. (2011) MamK, a bacterial actin, forms dynamic filaments in vivo that are regulated by the acidic proteins MamJ and LimJ. Mol. Micro. 82(2): 342-354

Gan, L., Ladinsky, M., and Jensen, G. J. (2011) Organization of the Smallest Eukaryotic Spindle. Current Biology 21(18): 1578-1583

Tocheva, E. I., Matson, E. G., Morris, D. M., Moussavi, F., Leadbetter, J. R., and Jensen, G. J. (2011) Peptidoglycan Remodeling and Conversion of an Inner Membrane into an Outer Membrane during Sporulation. Cell 146(5): 799-812

Reviewed by F1000 here

 

Shetty, A., Chen, S., Tocheva, E., Jensen, G. and Hickey, W. (2011). Nanopods: A new bacterial structure and mechanism for deployment of outer membrane vesicles. PLoS One 6(6):e20725

Reviewed in Nature Reviews Microbiology

 

Chen, S., Beeby, M., Murphy, G., Leadbetter, J., Hendrixson, D., Briegel, A., Li, Z., Shi, J., Tocheva, E., Muller, A., Dobro, M., and Jensen, G. (2011). Structural diversity of bacterial flagellar motors. EMBO 30(14): 2972-2981

 

Guerrero-Ferreira, R., Viollier, P., Ely, B., Poindexter, J., Goergieva, M., Jensen, G., and Wright, E. (2011). A unique mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus. PNAS 108(24): 9963-9968

 

Swulius, M., Chen, S., Ding, J., Briegel, A., Pilhofer, M., Tocheva, E., Lybarger, S., Johnson, T., Sandkvist, M., and Jensen, G. (2011). Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria. Biochem Biophys Res Commun 407(4): 650-655

Reviewed by F1000 here

 

 

 

2010

Fu, C. Y., Wang, K., Gan, L., Lanman, J., Khayat, R., Young, M. J., Jensen, G., Doerschuk, P. C., and Johnson, J. E. (2010). In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography. Structure 18(12):1579-1586

 

 

Tocheva, E., Li, Z., and Jensen, G.(2010). Electron Cryotomography. Chapter in Cold Spring Harbor Perspectives vol. 46 Bacterial Cell Biology, Lucy Shapiro and Richard Losick, editors.

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Pilhofer, M., Ladinsky, M., McDowall, A., and Jensen, G.(2010). Bacterial TEM: new insights from cryo-micrscopy. Methods in Cell Biology 96: 21-45

Jensen, G. J., editor (2010). Methods in Enzymology Vol 481: Cryo-EM, Part A, Sample Preparation and Data Collection. Elsevier Inc.

Micron Review Here

 

 

 

Jensen, G. J., editor (2010). Methods in Enzymology Vol 482: Cryo-EM, Part B, 3-D Reconstruction. Elsevier Inc.

Micron Review Here

Jensen, G. J., editor (2010). Methods in Enzymology Vol 483: Cryo-EM, Part C, Analyses, Interpretation, and Case Studies. Elsevier Inc.

Briegel, A., Chen, S., Koster, A. J., Plitzko, J. M., Schwartz, C. L., and Jensen, G. (2010). Correlated light and electron cryomicroscopy. Methods in Enzymology 481:317-341

Dobro, M., Melanson, L., Jensen, G., and McDowall, A. (2010). Plunge freezing for electron cryomicroscopy. Methods in Enzymology 481:63-82

Leong, P., Yu, X., Zhou, H., and Jensen, G. (2010). Correcting for the Ewald sphere in high resolution single particle reconstructions. Methods in Enzymology 482:369-380

 

Chen, S., McDowall, A., Dobro, M. J., Briegel, A., Ladinsky, M., Shi, J., Tocheva, E., Beeby, M., Pilhofer, M., Ding, H. J., Li, Z., Gan, L., Morris D. M., and Jensen, G. J. (2010). Electron Cryotomography of Bacterial Cells. JoVE. 39. doi: 10.3791/1943

CTPSIcon

Ingerson-Mahar, M., Briegel, A., Wener, J., Jensen, G., and Gitai, Z.. (2010). CTP synthase forms cytoskeletal filaments that regulate cell shape in Caulobacter crescentus. Nat Cell Biol 12: 739-746

Reviewed in A Metabolic assembly line in bacteria
Reveiwed by F1000 here

DipMIcon

Moll, A., Schlimpert, S., Briegel, A., Jensen, G., and Thanbichler, M. (2010). DipM, a new factor required for peptidoglycan remodeling during cell division in Caulobacter crescentus. Molecular Microbiology 77: 90-107

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Cabeen, M. T., Murolo, M. A., Briegel, A., Bui, N. K., Vollmer, W., Ausmees, N., Jensen, G., and Jacobs-Wagner, C. (2010). Mutations in the lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus. Journal of Bacteriology 192: 3368-3378

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Kuhn, J., Briegel, A., Morschel, E., Kahnt, J., Leser, K., Wick, S., Sogaard-Andersen, L., Jensen, G., and Thanbichler, M. (2010). Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. EMBO Journal 29: 327-339

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2009

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Iancu, C.V., Morris, D.M., Dou, Z., Heinhorst, S., Cannon, G.C., and Jensen, G.J. (2009). Organization, structure, and assembly of a-carboxysomes determined by electron cryotomography of intact cells. J. Mol. Biol. vol. 396(1) pp. 105-107

 

Briegel, Ariane; Ortega, Davi R.; Tocheva, Elitza I.; Wuichet, Kristin; Li, Zhuo; Chen, Songye; Muller, Axel; Iancu, Christina V.; Murphy, Gavin E.; Dobro, Megan J.; Zhulin, Igor B.; and Jensen, Grant J. (2009). Universal Architecture of Bacterial Chemoreceptor Arrays. PNAS (2009) vol. 106 (40) pp. 17181 - 17186

Reviewed by F1000 here

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He, Y., Jensen, G.J., and Bjorkman, P.J. (2009). Nanogold as a specific marker for electron cryotomography. Microsc Microanal (2009) vol. 15 (3) pp. 183-8

Li Current Opinion icon

Li, Z. and Jensen, G.J. (2009). Electron cryotomography: a new view into microbial ultrastructure. Current Opinion in Microbiology (2009) pp. 1-8

Suloway JSB 2009 icon

Suloway, C., Shi, J., Cheng, A., Pulokas, J., Carragher, B., Potter, C.S., Zheng, S.Q., Agard, D.A., and Jensen, G.J. (2008). Fully automated, sequential tilt-series acquisition with Leginon. J. Struct of Biol (2009) pp. 11-18

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Jensen, G. (2009). Cell biology. Protein filaments caught in the act. Science, vol. 323 (5913) pp. 472-3
Summary   Full Text

He Structure 2009 icon

He, Y., Jensen, G.J., and Bjorkman, P.J. (2009). Cryoelectron tomography of homophilic adhesion mediated by the neural cell adhesion molecule L1. Structure (2009) vol. 17 (3) pp. 460-71

   

2008

Gan PNAS icon

Gan, L., Chen, S. and Jensen, G.J. (2008). Molecular organization of Gram-negative peptidoglycan. Proc Natl Acad Sci USA, vol. 105 (48), pp. 18953-18957
CLICK here for movie 1 and movie 2

He Nature Icon

He, W., Ladinsky, M. S., Huey-Tubman, K. E., Jensen, G.J., McIntosh, J.R., Björkman, P.J. (2008). FcRn-mediated antibody transport across epithelial cells revealed by electron tomography. Nature, vol. 455 (7212) pp. 542-546

Ebersbach icon

Ebersbach, G., Briegel, A., Jensen, G.J. and Jacobs-Wagner, C. (2008) . A Self-Associating Protein Critical for Chromosome Attachment, Division, and Polar Organization in Caulobacter. Cell vol. 134 (6) pp. 956-968

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Lee, E., Fahimian, B., Iancu, C., Suloway, C., Murphy, G., Wright, E. R., Castaño-Díez, D., Jensen, G., Miao, J. (2008). Radiation dose reduction and image enhancement in biological imaging through equally-sloped tomography. Journal of Structural Biology, vol. 164, pp. 221-227

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Tivol, W.F., Briegel, A., and Jensen, G.J. (2008) . An Improved Cryogen for Plunge Freezing. MAM vol. 14 (05) pp. 5

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Briegel, A.B., Ding, H.J., Li, Z., Gitai, Z., Dias, P.D., and Jensen, G.J. (2008). Location and architecture of the Caulobacter crescentus chemoreceptor array by electron cryotomography Mol. Micro., available online.

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Morris, D.M. and Jensen, G.J. (2008). Towards a biomechanical understanding of whole bacterial cells. Ann. Rev. Biochem., 77:24.1-31.

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Yu, Z., Gonciarz, M.D., Sundquist, W.I., Hill, C.P., and Jensen, G.J. (2008). Cryo-EM structure of dodecameric Vps4 and its 2:1 complex with Vta1. J. Mol. Biol. 377:364-377

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Gavin E. Murphy, Eric G. Matson, Jared R. Leadbetter, Howard C. Berg and Grant J. Jensen Novel ultrastructures of Treponema primitia and their implications for motility Mol. Microbiol., 67 (6), 1184-1195

   

2007

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Murphy, G., Jensen, G.J. Electron Cryotomography BioTechniques Vol. 43, No. 4: pp 413-419 (Oct 2007)

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Osman, S., Moissl, C., Hosoya, N., Briegel, A., Mayilraj, S., Satomi, M., Venkateswaran, K. Tetrasphaera remsis sp. nov., isolated from the Regenerative Enclosed Life Support Module Simulator (REMS) air system International Journal of Systematic and Evolutionary Microbiology (2007), 57, 2749–2753

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Li, Z., Trimble, M.J., Brun, Y.V., Jensen, G.J. The structure of FtsZ filaments in vivo suggests a force-generating role in cell division. EMBO J. advanced online publication CLICK here for MOVIE

Reviewed by F1000 here

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Henderson, G.P., Gan, L., and Jensen, G.J. (2007). 3-D Ultrastructure of Ostreococcus tauri: Electron cryotomography of an entire eukaryotic cell. PLoS One 2(8): e749. doi:10.1371/journal.pone.0000749

Reviewed by F1000 here

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Iancu, C.V., Ding, H.J., Morris, D.M., Martino, A., and Jensen, G.J. (2007). The structure of isolated Synechococcus strain WH8102 carboxysomes as revealed by electron cryotomography. J. Mol. Biol. 372: 764–773. 

Reviewed by F1000 here

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Jensen, G.J. and Briegel, A. (2007). How electron cryotomography is opening a new window into prokaryotic ultrastructure.  Curr. Opin. Struct. Biol. 17:260–267.

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Wright, E.R., Schooler, J.B., Ding, H.J., Kieffer, C., Fillmore, C., Sundquist, W.I., and Jensen, G.J. (2007).  Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells.  EMBO J. 26: 2218–2226. Click here for MOVIE

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Jensen, G.J. (2007). A nanoengine for gliding motility (Invited Microcommentary). Mol. Microbiol. 63:4-6.

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Burns, D.G, Janssen, P.H., Itoh, T., Kamekura, M., Li, Z., Jensen, G.J., Rodriguez-Valera, F., Bolhuis, H., and Dyall-Smith, M.L. (2007).  Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain.  Int. J. Syst. Evol. Microbiol. 57:387-392.

 

2006

 
Iancu icon

Iancu, C.V., Tivol, W.F., Schooler, J.B., Henderson, G.P., Dias, D.P., Murphy, G.E., Wright, E.R., Li, Z., Briegel, A., Yu, Z., Gan, L., He, Yongning and Jensen, G.J. (2006).  Electron cryotomography sample preparation using the Vitrobot.  Nature Protocols, 1(6): 2813-2819

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Murphy, G.E., Leadbetter, J.R., and Jensen, G.J. (2006).  In-situ structure of the complete Treponema primitia flagellar motor.  Nature 442:1062-4. Click here for MOVIE

Reviewed by F1000 here

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Briegel, A., Dias, D.P., Li, Z., Jensen, R.B., Frangakis, A.S., and Jensen, G.J. (2006).  Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography.  Mol. Microbiol. 62(1):5-14.

Henderson icon

Henderson, G.P. and Jensen, G.J. (2006).  Three-dimensional structure of Mycoplasma pneumoniae's attachment organelle and a model for its role in gliding motility.  Mol. Microbiol. 60(2):376-385.

Wright icon

Wright, E.R., Iancu, C.V., Tivol, W.F. and Jensen, G.J. (2006).  Observations on the behavior of vitreous ice at ~82 and ~12 K  J. of Struct. Biol. 153:241-252.

Iancu icon

Iancu, C.V., Wright, E.R., Heymann, J.B., and Jensen, G.J. (2006).  A comparison of liquid nitrogen and liquid helium as potential cryogens for electron cryotomography.  J. of Struct. Biol. 153:231-240.

Komeili icon

Komeili, A.*, Li, Z.*, Newman, D.K., and Jensen, G.J.  (2006).  Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK.  Science, 311:242-245. (*denotes equal contibution)
Click here for MOVIE

Reviewed by F1000 here

 

2005

 
Murphy icon

Murphy, G.E. and Jensen, G.J. (2005).  Electron cryotomography of the E. coli pyruvate and 2-oxoglutarate dehydrogenase complexes.  Structure, 13(12):1765-1773.      *received "Exceptional 9.0 interest factor" in Faculty of 1000 reviews

Reviewed by F1000 here

Iancu icon

Iancu, C.V., Wright, E.R., Benjamin, J., Tivol, W.F., Dias, D.P., Murphy, G.E., Heymann, J.B., and Jensen, G.J. (2005).  A "flip-flop" rotation stage for routine dual-axis electron cryotomography.  J. of Struct. Biol. 151:288-297.

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Leong, P.A., Heymann, J.B., and Jensen, G.J. (2005).  Peach: A simple Perl-based system for distributed computation and its application to cryoEM data processing.  Structure, 13:1-7.

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Benjamin, J., Ganser-Pornillos, B.K., Tivol, W.F., Sundquist, W.I., and Jensen, G.J. (2005).  Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography.  J. Mol. Biol. 346(2):577-588.      *one of top ten downloaded JMB papers in 2005

Reviewed by F1000 here

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Ackerson, C.J., Jadzinsky, P.D., Jensen, G.J., and Kornberg, R.D. (2005).  Rigid, specific, and discrete gold nanoparticle/antibody conjugates.  J. Am. Chem. Soc. 128:2635-2640.