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Members of the Jensen Lab are the primary users of the Cryo-Electron Microscopy Facility at the Broad Center.
Here we have access to state-of-the-art equipment, the most sophisticated of which is the FEI Polara (pictured left). The Polara is a 300kV TEM equipped with an FEG, a Gatan Image Filter and a Gatan Ultracam, which is a 4k by 4k, lens-coupled, cooled CCD camera. This microscope is also equipped with the Leginon Automated data collection software suite as well as the UCSF Tomo automated tomography software. Other imaging equipment includes a Tecnai T12 TEM, equipped with a Gatan Ultrascan 2k by 2k CCD and Serial EM automated software, as well as a Nikon 90i computer-controlled epi-fluorescence microscope with Metamorph automation & imaging software. The facility also has all the equipment needed for the preparation of both frozen-hydrated and freeze-substituted samples. Plunge-freezing of frozen-hydrated samples is done on either the FEI Mark III Vitrobot or an EMBL-style gravity-plunger. Cryo-ultramicrotomy is made possible with the Leica ultracut UCT, the Balzer High-pressure-freezer and the Leica EMFS freeze-substitution machine. The lab also has a full assortment of equipment for microbiology, biochemistry and molecular biology, as well as access to the many excellent core facilities available at Caltech. |
